الفهرس | Only 14 pages are availabe for public view |
Abstract Maha Sayed Ali Haridy Hassan: Effect of some Biocides and Chemicals on Tomato Genome and its Resistance to Some Pathogens under Green House and Tissue Culture Technique. Unpublished PhD. Thesis, Department of Genetics, Faculty of Agriculture, Ain Shams University, 2016. Tomato (Lycopersicon esculentum Mill.) is one of the most important economic vegetable crops in Egypt. This work aims to studythe effects of two biocides; clean root with active ingredients; Trichoderma harzianumor or Bacillus subtilis and two fungicides; Rizolex or Topsen which used to control damping off disease caused by two pathogens; Fusarium solani and Rhizoctonia solani on the behavior of meiosis, pollen grains fertility, structure and morphology and callus growth variation of the tomato cultivars. Evaluation of 12 tomato cultivars against the two pathogens infection revealed that the most susceptible cultivar was Castle rock, the most resistant cultivar was Nemathoda and the moderately susceptible cultivar was super strain B. The use of the two fungicides was more effective in control pathogens than the use of the two biocides; individually or in dual application on tomato cultivars except cultivar Nemathoda for control of F. solani, which showed no significant differences among fungicides and biocides treatments compared to the controls. Cytogenetic analyses of meiotic division, the genome (2n) of pollen grains (PG) and their fertility and morphology showed that fungicides were more effective on meiotic division aberrations than the other treatments with three concentrations for the three cultivars. Castle rock cultivar showed the highest damage for the behavior of cells during meiosis with fungicides application. Biocides applications exhibited less damages than the fungicides. At the level of tissue culture, results recommended the use of bioagents in control the infected pathogens on tomato plants than fungicides application. Keywords; Tomato, bioagent, biocides, chemical fungicide, tissue culture, culture filtrates, cytogenetics, pathogens. |