الفهرس 
Methodology
PreeclampsiaOnly 14 pages are availabe for public view
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Abstract
The association between periodontitis and some of the
problems with pregnancy such as premature delivery, low
weight at birth, and preeclampsia (PE) has been suggested .
This case-control study was done at Ain Shams University
Maternity Hospital, between Aprill 2015 and Septemper 2015;
to study the association between Porphyromonas gingivalis and
Preeclampsia .A total of 48 patients, having single intra-uterine
fetus, were included in the study . Subjects were selected from
those attending the delivery unit and were scheduled for elective
cesarean delivery.They were divided into 2 groups;24 patients
in each . Group (1) were suffering from preeclampsia and
group (2) were normotensive . Pregnant women with any
associated medical disorder and patients who used systemic
antimicrobial therapy were excluded from the study .
History and oral examination:
A full history was taken from each patient and oral
examination was performed for each patient by a single
periodontist prior to cesarean delivery. Participants were
grouped according to periodontal status into ; normal healthy
gingiva ,gingivitis and periodontitis . Specimens :
Sub-gingival plaque and placental samples were collected
from each patient and transported to polymerase chain reaction
(PCR) section within 4 hours of collection . The samples were
stored at -60°C until PCR analysis . Total DNA from placental
and subgingival samples was extracted by using the QIAamp
Tissue Kits (Qiagen, Hilden, Germany ) according to the
manufacturer’s instructions .
Ethical approval :
The study was approved by ethical and research committee
of council of Obstetrics and Gynaecology Department ,Ain
Shams University at April 2015 .
Results:
There were no significant statistical differences between the
two groups as regards maternal characteristics. But, there were
significant statistical differences between the two groups as
regards neonatal birth weight which was lower in preeclmpsia
group .
There were significant statistical differences between the
two groups as the proportion of women who had positive
P.gingivalis in placental samples were higher in preeclampsia
group (group 1) when compared to the normotensive control
group (group 2) with statistical significance .The proportion of women who had positive P.gingivalis in
subgingival plaque samples were higher in group 1 when
compared to group 2 ; however the difference did not reach a
statistical significance . There was only mild agreement
between the two samples .
The proportion of women who had positive P.gingivalis in
placental samples were higher in patients with middle and low
socioeconomic status than patients with high socioeconomic
status in group 1.
These results point out that Porphyromonas gingivalis
may be a precipitating factor or an aggravating factor but not a
sole etiological factor in the development of preeclampsia .