الفهرس 
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Abstract
Incidence of Vibrio Parahaemolyticus in Marketed Marine Fish
A total of 300 market marine fish, 150 each of Seabass (Dicentrarchus labrax) (50 raw fish, 50 grilled ready-to-eat and 50 fried ready-to-eat from the same market) and 150 of Seabream (S. aurata) (50 raw fish, 50 grilled ready-to-eat and 50 fried ready-to-eat from the same market), were collected from Alexandria governorate and examined microbiologically for Vibrio parahaemolyticus occurrence of Vibrio parahaemolyticus. The Seabream Sparus aurata was the highest infected species 50% followed by Seabass Dicentrarchus labrax 34%. Incidence rate of Vibrio parahaemolyticus grilled ready-to-eat Seabass Dicentrarchus labrax and Seabream (S. aurata) 0% and in fried ready-to-eat 0%. The phenotypic charachterization of Vibrio parahaemolyticus revealed that the colonial appearance of on TCBS, showed swarmed green colonies. The gram staining showed pleomorphic straight or curved rods. Biochemical identification of Vibrio parahaemolyticus showed that they are gram negative, motile, cytochrome oxidase and catalase positive, sensitive to vibriostatic agent pteridine (O/129, 150 μg) and On Sheep blood agar with 3% NaCl give β hemolytic patterns. The confirmatory diagnostic API-20E system of Vibrio parahaemolyticus resulting positive in these tests. The biochemical identification of marine strains may be a suitable method but however, the results may value up to genus level and not to species level. The species- specific detection of V.parahaemolyticus was performed using single PCR assay. V.parahaemolyticus have yielded amplification products of expected molecular size 368 bp.