Search In this Thesis
   Search In this Thesis  
العنوان
Studies on using of Molecular Diagnostic Methods for Identification of Staphylococcus aureus
المؤلف
Ibrahim, Asmaa Mohammed Baracat.
هيئة الاعداد
باحث / Asmaa Mohammed Baracat Ibrahim
مشرف / Alaa El-Din Hussein Mustafa
مشرف / Rasha Nabil Zahran
مشرف / Rasha Nabil Zahran
الموضوع
Superantigens. Bacterial antigens. Superantigens - physiology. Antigens, Bacterial - physiology.
تاريخ النشر
2015.
عدد الصفحات
88 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الفيروسات
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة مدينة السادات - كلية الطب البيطري بالسادات - Department of Bacteriology,
الفهرس
Only 14 pages are availabe for public view

from 111

from 111

Abstract

In this study 100 milk samples were collected from dairy farm and
random field samples in Menofia governorate/ for investigation the
prevalence of Staphylococcus. aureus from clinical mastitis in cattle and
buffaloes. The clinical cases were diagnosed by clinical examination of
the udders as the cardinal signs of inflammation (redness, edema, loss of
function, pain and hot) only 65 samples were present (34 from cattle and
26 from buffaloes. The prevalence of mastitis among animals quarters
were found in cattle as fore quarter found in 18 samples ( 52.94%) and
hind quarters found in 16 samples (47.05%%) and buffaloes as fore
quarter found in15 samples (57.69%) and hind quarters found in11
samples (42.30%.) Isolation of Staphylococcus aureus on mannitol salt
agar medium from collected samples in cattle 34samples gave 28 positive
growth (82.35 %), and negative growth 6 samples ( 17.65 %) and in
buffaloes 26 samples gave 12 positive growth (46.15%) , negative 14
samples (53.85 %). Biochemical reaction of S. aureus on catalase test all
the isolates gave positive results from cattle and buffaloes mastitis
samples. While coagulase test gave 13 isolates (46.43%) in cattle and 4
isolates (33.33%) in buffaloes. The isolated S. aureus tested for pigment
production on mannitol salt agar medium which the positive isolates gave
golden yellow pigment on the medium. In cattle were 8 isolates (28.57%)
and 20 isolates (71.43%) for positive and negative respectively. and in
buffaloes 4 isolates (33.33%) and 8 isolates (66.67%) for positive and
negative respectively.