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Abstract In this study 100 milk samples were collected from dairy farm and random field samples in Menofia governorate/ for investigation the prevalence of Staphylococcus. aureus from clinical mastitis in cattle and buffaloes. The clinical cases were diagnosed by clinical examination of the udders as the cardinal signs of inflammation (redness, edema, loss of function, pain and hot) only 65 samples were present (34 from cattle and 26 from buffaloes. The prevalence of mastitis among animals quarters were found in cattle as fore quarter found in 18 samples ( 52.94%) and hind quarters found in 16 samples (47.05%%) and buffaloes as fore quarter found in15 samples (57.69%) and hind quarters found in11 samples (42.30%.) Isolation of Staphylococcus aureus on mannitol salt agar medium from collected samples in cattle 34samples gave 28 positive growth (82.35 %), and negative growth 6 samples ( 17.65 %) and in buffaloes 26 samples gave 12 positive growth (46.15%) , negative 14 samples (53.85 %). Biochemical reaction of S. aureus on catalase test all the isolates gave positive results from cattle and buffaloes mastitis samples. While coagulase test gave 13 isolates (46.43%) in cattle and 4 isolates (33.33%) in buffaloes. The isolated S. aureus tested for pigment production on mannitol salt agar medium which the positive isolates gave golden yellow pigment on the medium. In cattle were 8 isolates (28.57%) and 20 isolates (71.43%) for positive and negative respectively. and in buffaloes 4 isolates (33.33%) and 8 isolates (66.67%) for positive and negative respectively. |