الفهرس 
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Abstract
In this study, a total number of 100 meat samples were collected from two different governorates (Giza and Kalubia) for the isolation and typing of Staphylococci. Samples were obtained either from butchers shops (chicken, local beef meat) and supermarkets (imported frozen). The meat samples consisted of 50 chicken meat samples (19 breast meat, 21 thigh meat and 10 livers), 27 local beef meat and 23 imported frozen beef meat. The incidence of isolation of Staphylococcus spp. was 100% from different meat samples. The majority of Staphylococcus isolates showed slime formation (40 %) and biofilm formation (85%). The prevalence of β-hemolysis activity of Staphylococcus spp. reached 66% while α-hemolysis and γ–hemolysis activities reached 18% and 16% respectively. All Staphylococcus isolates supernatant fluid showed cytopathic effect on the Vero cell line (100%). The results of antimicrobial disk diffusion test of the isolated Staphylococcus spp. revealed that most of them were highly resistant to clindamycin, erythromycin, gentamicin, penicillin, sulfamethazole/trimethoprim and tetracycline (100% resistant). All the isolated Staphylococcus spp. (100 isolates) were positive for the amplification of the 16S rRNA gene (specific for genus Staphylococci) with an incidence of 100%. For genotypic detection of different antibiotic resistance genes of the isolated Staphylococcus spp., revealed that the amplification for mecA was expressed in 35 of the isolated Staphylococci (35%) and the amplification of the gyrA, gyrlA and gyrB genes was detected in a percentage of 60%, 57% and 33% respectively, while The prevalence of a cfr gene was in a percentage of 5%. The high frequencies of virulence traits present combined with the high antimicrobial co-resistance profiles observed, suggest that these isolates may represent a serious problem with major public health implications, re-enforcing the importance of the wide concept of “One Health”.