الفهرس 
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Abstract
The present work was designed to study the ultrastructure and possible defects that may be induced during the freezing of buffalo bull spermatozoa.
Fresh semen samples were collected from three buffalo-bulls from Mahallet Musa buffalo research station in Kafr El-shiekh Government for production of buffalo-bull sires. The semen was evaluated considering; volume, color, viscosity, pH, percentage of mass and individual motility, sperm abnormalities and sperm cell concentration per mm3. Straws of frozen semen were obtained from Kafr El-Shiekh Center for frozen buffalo-bull semen. Six mini straws were used for making three resin blocks. All these samples were prepared for examination by transmission electron microscope.
The results reveal that the Spermatozoa of buffalo bull consist of: head and tail. The tail is the longest part of the sperm. The tail attachment to the head is axial in position and consists of neck, middle piece, principle piece and end piece.
The sperm head consists of the acrosome and the nucleus being surrounded by the plasma membrane. The acrosome forms a cap-like structure covering the first two thirds of the sperm head. The nucleus contains the condensed chromatin usually flattened dorsoventrally. There is a small area of uncondensed chromatin where transcription, translation and protein-biosynthesis are still possible. The concave implantation fossa is at the caudal end of the nucleus and it is lined by nuclear envelope.