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Abstract SUMMARY Despite being recognized for many years, needle stick and sharp injuries (NSSIs) continue to present a risk of occupational exposure of HCWs to blood-borne pathogens. This study was conducted to investigate the epidemiological characteristics of NSSIs among HCWs, immunization status for hepatitis B virus (HBV) and prevalence of serological markers of HBV and HCV in Alexandria Main University hospitals. It was a retrospective survey of all self-reported documents related to NSSIs, during the period of 7 years from 2005 to 20011. This study included 90 HCWs who reported NSSIs, nurses represented 63.3% auxiliary staff 33.3%, while physicians represented only 3.3% of study population. This study revealed that: 1. Increase in the reported cases of NSSIs from 7 injuries in 2005 to 25 during the year 2011. 2. More injuries occurred in medical wards (53.3%) than in more intense areas such as the emergency rooms. 3. Source patient was identified in only 62.2% of cases, while in 37.8% the source patient was unidentifiable. 4. In 34(37.8%) of injuries HCW was not the original user of the sharp instrument. 5. More injuries (52.2%) occurred after use of the sharp item than during the procedure (47.8%). 6. HBV vaccination coverage was 32.2%. 7. Auxiliary staff group had the lowest vaccination coverage, only (16.7%) were vaccinated. 8. Prevalence of anti-HBc antibodies, HBsAg and anti-HCV antibodies were 17.8%, 2.2% and 12.2% respectively. 9. Overall prevalence of viremic hepatitis C and B as confirmed by PCR were |