الفهرس 
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Abstract
Many cellular functions are regulated by changes in gene
expression. Thus, quantification of transcription levels of genes plays
a central role in the understanding of gene function and of abnormal
alterations in regulation that may result in a disease state. There is
resurgence in the use of herbal medicines worldwide.
In contrast to chemical drugs, herbs have sometimes been claimed to
be non-toxic, because of their natural origin and long-term use as
folk medicines. Using of thymol in form of adjuvant induce the
immune response and cytokines production.
Therefore, the outcome of this work was to demonstrate the
feasibility of using some physical treatments on MDR E. coli 026
and follow up the gene expression of some proin:flammatory
cytokines and toll-like receptors-4 ex-vivo.
In the present work, The susceptibility pattern of E. coli 026
revealed that it was sensitive to cefoperazone (CFP), cefuroxime
(CXM) , ciprofloxacin (CIP) ,gentarnicin(CN), irnipenem (lpm) and
amikacin (AK) and while it showed resistant to more than three
antibiotics Nitrofurantoin (F), Streptomycin (S), Tetracycline (TB),
Trimethoprime/sulphamethoxazole (SXT) and Ampicillin
/Sulbctarn (SAM), hence meeting the criteria for multidrug
resistant.
Regarding the calculation of Colony forming units (CFU) of E.
coli 026 strain, it was determined and the bacterial cell count was
36x IOII/rn!.
Concerning the inoculation of rabbits with MDR E. coli 026 strain
in different physical treatments within different time points, marked
gene expressions of TLR-4 and some proin:flammatory cytokines
mRNA was revealed.