الفهرس 
Introduction and Aim of the WorkOnly 14 pages are availabe for public view
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Abstract
Staphylococcal isolates are among the most frequent isolates from wound infections. They cause several infections such as, post operative surgical site
infection, diabetic leg ulcer infection, abscess discharge, and burn infection. This study was carried out to detect the causative agent of wound infections in some hospitalized patients in MUH, determine the antimicrobial susceptibility pattern of the isolated Staphylococci strains, detect the prevalence ofMRSA among the isolates. and examine the possible genetic origin of methicillin resistance among the isolated MRSA strains.
In the present study, a total of 208 samples were collected from SSTls from patients attending MUH from November 2007 until August 2010. In this study. the highest number of samples was collected from 82 post operative surgical site infection (SSI) patients (39.4% of the total samples) followed by 52 diabetic foot ulcer and 45 abscess discharge swabs representing 25% and 21.6%) of the total samples. respectively. Finally, 29 burn infection samples (13.9%) were also collected.
A total of 372 isolates were retrieved, in which Gram-positve bacteria were the commonest organisms (272 isolates) found in wound infections (73.1 %), with a predominance of polymicrobial infections in 157 subjects (75.5%), 37 monomicrobial samples (17.8%) and 14 culture negative samples (6.7%). A total of241 Staphylococcal species (64.8%) represented the most commonnly isolated pathogen. Out of the 241 infected isolates, 127 S. aureus isolates (61% of the total patients and 53% of the staphylococcal isolates) were detected and 114 isolates (55% of the total patients and 47% of the staphylococcal isolates) were coagulase negative Staphylococci (CoNS). The other identified pathogens were 31 Sterptococcal species
(14.9%),15 Pseudomonas isolates (7.2%), 9 Proteus isolates (4.3%), and other Grammnegative species were 76 isolates representing 36.5% of the total patients.
The highest prevalence of S. aureus was observed in patients of age group 41 P60 years (73.7%), followed by 21- 40 (54.3%). Patients aged >60 years and < 20 years had 45.2 and 41.7% prevalence, respectively. On the other hand, the highest prevalence of CoNS was observed in patients in the age group>60 years (71 %), followed by patients of age group between 21- 40 years (55.7%), while the lowest prevalence was observed in patients of age group between 41- 60 years age group and < 20 years (50.5% and 41.7%, respectively).
S. aureus strains were isolated from males and females at almost the same
ratio (61 %). The prevalence of CoNS was higher in females (73 .8%) than in males (46.2%).
The highest prevalence of S. aureus was observed in diabetic foot ulcers at a rate 01’75% (39 isolates) followed by post surgical wound infection 58.5% (48 isolates) and abscess swabs 55.6% (25 isolates) then burn discharge 51.7% (15 isolates ). On the other hand, the highest prevalence of CoNS was found in abscess discharge 66.7 % (30 isolates) followed by surgical wound infection 54.9 % (45 isolates) then diabetic foot ulcers 50% (26 isolates) and finally burn swabs 44.8% (13 isolates ).
The isolated bacteria were screened for their susceptibility to different antibiotics using agar dilution technique. For better comparison, MIC9o, MICso, the number and percentage of isolates sensitive. intermediate sensitive and resistant to each antibiotic were recorded and showed that the isolated S. aureus strains showed low resistance to vancomycin (1.6%), amikacin (2.4%), gatifloxacin (4%), and levofloxacin (8.7%). Moderate resistance to gentamicin (17.3%), oxacillin (24.4%), Ofloxacin (24.4%).