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Abstract Since fish and shellfish are subject to the risk of contamination with various pathogens either during their presence in their aquatic environments or after being harvested for marketing, this work was done to ass sanitary conditions of Mugil cephalus, sardine, shrimp and Om El—Kholoul caught from natural sources. One hundred samples of Mugil cephalus, sardine, shrimp and Om El—Kholoul (25 of each) collected from different markets of Kafr El—Sheikh governorate were subjected to bacteriological examination. The bacteriological examination indicated that: Vibrio parahaemolyticus was isolated from Mugil cephalus, sardine and shrimp at an incidence of 32, 24 and 24%, respectively with mean values The isolated halophilic bacteria could be identified as Moraxella, Flavobacterium, Acinetobacter, Pseudomonas mallei and Pseudomonas pseudomallei at varying percentages from the examined samples of Mugil cephalus, sardine, shrimp and Om El-Kholoul on different media (SWA and TSA) and different incubation temperatures (37 and 7 °C). On the other hand, other identified isolates such as Aeromonas, enterobacteria, Bordetella bronchioseptica and Chromobacterium hridum could be isolated at varying percentages from the examined samples of Mugil cephalus, sardine, shrimp and Om El-Kholoul on different media (SWA and TSA) and different incubation temperature (37 and 7 °C). The public health significance of the isolated bacteria was discussed and the suggestive measures to protect the consumer as well as production of high quality fish and shellfish were discussed. |