الفهرس 
MESENCHYMAL STEM CELLSOnly 14 pages are availabe for public view
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Abstract
MSCs are a heterogenous population of self renewable, pluripotent
cells that can be isolated from bone marrow and other sources. They are
capable of restoring the hematopoietic microenvironment after backtransplantation
of even a single clone into the body.
The aim of this study was to examine the effect of local delivery of
MSCs on muscle regeneration in a murine limb ischemia model. To achieve
this aim, rat hindlimb ischemia model was established by surgical ligation of
the left femoral artery. Animals were grouped; control rats, ischemic group
and, ischemic with hMSCs group. In vitro, hMSCs were isolated from
human bone marrow and characterized by flow cytometry. hMSCs were
labeled by red fluorescent PKH dye. Peak isometric twitch force (Pt), was
assessed 4 weeks after surgery. After rats were sacrificed, muscle tissues of
the three studied groups were harvested for pathological assessment, tissue
tracing of labeled MSCs and for vascular endothelial growth factor gene
expression using quantitative real time PCR. Our results showed that hMSCs
were positive for mesenchymal stem cell marker. Histopathologically,
ischemic muscle transplanted with hMSCs showed definite angiogenesis&
neovascularization. Red fluorescent PKH dye for best cell tracing showed
that the red fluorescence was found in ischemic muscle injected with
hMSCs. Ischemic with injected hMSCs group induced a significant
improvement in blood reperfusion, detected by improved muscle
performance, high significant level of VEGF gene expression compared to
ischemic group.
Key words: rat hindlimb ischemia - hMSCs – VEGF- skeletal muscle -
angiogenesis.