الفهرس | Only 14 pages are availabe for public view |
Abstract The thesis is devoted to spectrophotometric, chromatographic and electrophoretic analysis of antihypertensive drugs which has an urgent need in the pharmaceutical field nowadays, as well as the crucial importance of the analysis of enantiomers as almost most of drugs in use are chiral. Four antihypertensive drugs namely Amlodipine besytale (AML), Perindopril Erbumine (PER), Valsartan (VAL) and Hydrochlorothiazide (HCT) have been determined via this approach. The thesis comprises four parts: Part I. General introduction This part provides a general introduction about hypertension and an analytical review for the analytical techniques used for the determination of the studied drugs either in dosage forms or in biological fluids. Part II: This part consist of four sections Section (A): The suggested method based on measuring the peak amplitude of the first derivative of the ratio spectra for the simultaneous determination of amlodipine besylate and perindopril erbumine, each peak amplitude was measured at the specified wavelength. Section (B): This method develops a stability indicating ion pairing isocratic reversed phase HPLC method for the simultaneous determination of binary mixture of AML besylate and PER erbumine in presence of their degradation products. The efficient separation was achieved on Zorbax C18 analytical column using mobile phase consisting of 0.05 M phosphate buffer: acetonitrile in the proportion of (30/70, v/v) with the pH adjusted to of 3.0 with orthophosphoric acid. Section (C): TLC-densitometric method was developed for the simultaneous determination of binary mixture of AML besylate and PER erbumine. The efficient separation and identification were achieved on utilizing n-butanol: water: acetic acid in the ratio (4:5:1by volume) as a mobile phase. Section (D): Development HPLC method simultaneous determination of AML besylate, VAL and HCT in bulk powder, laboratory prepared mixtures and its tablet dosage form. Part III: This part consist of three sections Section (A): The suggested HPLC method based on separation of S-PER in presence of its R-enantiomer using of β-cyclodextrin as a chiral selector through ChiraDex chiral column. The efficient separation and identification were achieved using acetonitrile and 0.05 M potassium dihydrogen phosphate solution with the pH adjusted to of 3.0 with orthophosphoric acid in the proportion (45:55 v/v) as a mobile phase. Section (B): The proposed CE method based on separation of S-PER in presence of its R-enantiomer using Hydroxypropyl β- cyclodextrin as a chiral selector. Different factors affecting electrophoretic mobilities were studied such as buffer type, pH, chiral selector, organic modifier and analytical voltage. Summary and Conclusion |