الفهرس 
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Abstract
This investigation will be conducted into five phases. In experiment (1), using of 0.5 g camel milk whey powder with Leiurus species venom in horses were resulted in increase the antisera potency to 78.3 LD50 in comparison to control group (49.2 LD50). The same were done with N. nigricollis venom in horses with 0.5 g camel milk whey powder; increase the antisera potency to 65 LD50 in comparison to control group (45 LD50) .Also, using of 0.5 g cow milk whey powder with Leiurus species venom in horses resulted in increasing the antisera potency to 63.3 LD50 in comparison of control group (45 LD50). The same were done with N. nigricollis venom in horses with 0.5 g cow milk whey powder, increase the antisera potency to 55.8 LD50 in comparison of control group (42.5 LD50). In experiment (2), using of 5 mg of pure bovine lactoferrin with Leiurus species venom in horses were resulted in increase the antisera potency to 90 LD50 in comparison of control group (49.2 LD50). The same were done in N. nigricollis venom in horses with 5 mg of pure bovine lactoferrin, increase the antisera potency to 75 LD50 in comparison of control group (50 LD50).In experiment (3), using of complete Freund’s adjuvant (CFA) with Leiurus species venom in horses resulted in increase the antisera potency to 80 LD50 in comparison of control group (50 LD50). The same were done in N. nigricollis venom in horses with (CFA); increase the antisera potency to 65 LD50 in comparison of control group (49.2 LD50). Studying of immune effect of bovine lactoferrin through Lymphocyte Transformation Test (LTT) was done. Phytohaemaglutinin (PHA) used as a control without lactoferrin, the lymphocyte transformations mean value was 2.37± 0.06. The different concentration of lactoferrin (in conc. of 10ug/ml, 20ug/ml and 50ug/ml) without phytohaemaglutinin (PHA), lymphocyte transformations mean values of lactoferrin were 1.805± 0.040, 1.955± 0.045 and 2.39± 0.053 respectively. While different concentration of lactoferrin (in conc. of 10ug/ml, 20ug/ml and 50ug/ml) with phytohaemaglutinin (PHA), lymphocyte transformations mean values were 2.12± 0.03, 1.941± 0.024 and 1.861± 0.1 respectively. The lymphocyte transformations mean values (LTT) were 3.076±0.09 and 2.49±0.05 for experiment group (5) (horses were injected with Naja nigricollis venom with lactoferrin) and control group (5) (horses were injected with Naja nigricollis venom without lactoferrin), respectively. Camel and cow milk whey were used as a source of Lf in a crude form. The Sodium dodecyl sulfate – Poly Acrylamid Gel Electrophoresis (SDS-PAGE) profile analysis reveals that, milk whey samples had a band at 80 kDa.