الفهرس 
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Abstract
In this study, an ELISA was developed for detection of antibodies
against BHV -1 antibodies BHV -1. Colorado strain of BHV -1 was propagated
on MDBK cell and characterized by using SNT and FAT. The virus was
titrated after and before concentration by polyethylene glycol (PEG). The
protein contents of the concentration virus were determined. In the
optimization steps of ELISA kit the best results obtained when antigen
concentration used (Sug/ml) and in 1/25 dilution of serum were used. The
efficiency of the prepared ELISA was studied by comparison the results with
those of SNT when applied both tests on 86 serum samples. Eight five % of
these samples were positive by ELISA and only 61 % were positive by SNT.
Comparison between the prepared ELISA and SNT as well as commercial
ELISA kit was carried out. A slight difference was reported. Application of
the prepared ELISA kits was employed on the screening of 354 serum
samples collected from 5 different localities, El Behira (vaccinated), Sharkia,
Menofia, El Fayoyum and Kafr El Sheik. The prevalent percent of anti BHV-
1 antibodies were 95, 88, 80, 38, and 48 respectively. Also analysis of the
obtained ELISA results revealed that samples of cattle were high prevalence
percentage than those of buffalo. In conclusion, the present study reports the
development of a locally prepared ELISA hits for detection of anti BHV-I
antibodies and the success of these kits in the field application trial.