الفهرس 
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Abstract
This study presents a trail for preparation of AI-ND and Al vaccines and study the duration and
level of immunity induced by such vaccines under experimental condition. The serological tests
carried out on serum samples obtained from vaccinated chickens with single and combined Al and ND
vaccines revealed that such birds exhibited detectable AI antibodies by the first week post vaccination
with single AI and combined Al and ND vaccines of a titer 6± 1.15 & 2± 1.0 by HI test and
0.4S1±0.217 & 0.323±0.19S by ELlSA; respectively. The peak of AI antibody titer was induced by the
monovalent vaccine (8.7± 1.26 by HI test and 1.067±0.4S0 by ELISA) by the third week while the
combined vaccine recorded such peak (7.5 ±0.S8 by HI test and 0.949±0.669 by ELlSA) by the
seventh week post vaccination. Detectable ND antibodies were detected in vaccinated chickens with
titers of 6.7±1.IS & 6.S±I.0 by HI test on. the first week post vaccination with single ND and
combined AI and ND vaccines respectively. These antibodies reached their peak (8.7 ±1.26& 7.S±O.S8
by HI at the third and eleventh week post vaccination with single and combined vaccines; respectively
The study reports the first trail for Preparation of a combined inactivated Vaccine to Avian
Influenza and Newcastle disease viruses.