الفهرس 
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Abstract
Chicken rotaviruses (CRVs) were detected for the first time in Egypt. Isolation, identification and molecular characterization of chicken rotaviruses were performed in our current studies. We collect and screened fecal samples from 85 naturally occurring diarrhea outbreaks in commercial chicken broilers farms that located in a wide range of geographical area distribution including many Egyptian governorates mainly (6th October, El Fayom, Giza, Kaleobea, Menofia and El Mansoura) during 2008. Chicken RV was detected in the fecal samples by performing different laboratory and confirmatory tests including ELISA using monoclonal antibodies against VP6 , Electron Microscopy (EM), Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Isolation in SPF chickens. The obtained results confirmed the isolation and identification of group A chicken rotavirus while the molecular characterization analysis using different primers sets proved that the isolated chicken rotavirus not belongs to the same cluster of Ch1 rotavirus strain, more related to Po-13 strain and confirm that it belongs to mammalian like chicken rotavirus strains. The results also show that in the assessed broiler chicken flocks, chicken RVs was directly related to diarrhea observed in broiler chickens up to a month old. As no studies have been published yet related to chicken rotaviruses in Egyptian poultry. This study is considered as the first record study not only succeeded to isolate avian rotavirus for first time in Egypt but also discuss the importance of rotaviruses in broiler chickens with delayed growth and diarrhea.