الفهرس 
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Abstract
In the present study 4 types of media were tried for production of tetanus toxin namely : M&M , 74A, M&M, 74B, M&M, 54 and LPF, 61. These media were used for both standard Harvard strain and one of local isolates. The last medium gave the highest toxigenicity in mice where the MLD reached 800,000 /ml and antigenicity 80 LF/ml and 75 KF. The role of uracil in toxin production was also studied. Detection of uracil from both media (M&M, 54 and LPF, 61) caused a sharp DROP in the produced toxin potency. Deletion of uracil from LPF, 61, resulted in reduction of the toxigenicity of the produced toxin from 800,000 MLD/ml to only 1000 MLD/ ml, while the flocculation decreased from 80 LF and KF from 75 min to zero KF. Also M&M, 54 medium showed reduction in toxigenicity and antigenicity from 400,000 MLD/ml to 10,000 and from 60 LF to zero, KF from 95 min. to zero, respectively when uracil was deleted. Also, isolation and identification of C. tetani from faecal swabs and deep lacerated wounds of horse was carried out. Isolation of 2 field isolates from faecal swabs only and confirmation was done by colonial appearance, cultural characters, biochemical tests and neutralization test. In conclusion a potent tetanus toxin was produced from protein free medium using a standard strain (Harvard), while a much lesser potent toxin was harvested from the locally isolated strain.