الفهرس 
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Abstract
The objective of the current study is to develop an optimized PCRbased
detection method for the Egyptian isolates of the infectious
bursal disease virus (IBDV-E). To this end, four PCR primers based on
published IBDV sequences determined throughout the world were
synthesized commercially and used to amplify the hypervariable region
(HVR) in VP2 gene.
The results of this study could be summarized as follows:
The integrity of the RNA purified from 0.1g bursa was maintained
throughout RNA preparation as confirmed by agarose gel
electrophoresis. The amplification of IBDV sequence by the outer and
inner primers was optimal at annealing temperature of SO°Cin both the
primary and secondary PCRs. The suitable concentration of MgCIz for
RT and PCR was found to be 2.5 mM.
Amplification of IBDV sequences from infected bursa by RT-PCR
was performed on total RNA extracted from 0.1g bursal tissue. The size
of the amplified DNA product (144 bp) was in agreament with that
expected from the published nucleotide sequence of the IBDV VP2 gene.