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Abstract
Increasing numbers of elderly people are being treated in hospitals and are additionally at particular risk of acquiring nosocomial infections (NI) with antibiotic-resistant organisms.
Prevalence of NI had been evaluated in this study in (100) inpatients in Geriatric Medicine Unit, EL Demerdash Hospital of Ain Shams University from March 2004 till September 2006. The association of NI with intrinsic and extrinsic risk factors was also assessed, as well as the emergence of antibiotic resistant bacterial strains.
All specimens were inoculated onto blood agar, chocolate agar, MacConkey agar and nutrient agar and for urine specimens were inoculated onto blood agar, MacConkey agar and nutrient agar and incubated aerobically at 37 oC for 24-48 hours. We identified the isolated organisms by colony morphology, Gram staining, microscopical examinations and biochemical reactions according to the Clinical and Laboratory Standards Institute (CLSI).