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Abstract some fungi have been isolated from the root of some healthy and BSSD plants. fungal content of both the rhizosphere and rhizoplane of the |plants was compared with those of the healthy ones. In this r>pes of plants. ic causal fungal pathogen of tomato wilt was isolated and VAhernaria alternata and Penicillium digitatum were isolated from ^tomato and orange fruits respectively. Some Egyptian plants have been studied as controlling agents of plant fungal diseases. In our present study, we found that Vonum salicifolium has good results in this regard. The crude extract wygonum was found to have an inhibitory activity against some of jpaihogenic fungi Fractionations of the crude Polygonum extract have been made Solvents of increasing polarity. The fractionation leads to a partition ^ extract into four parts according to each solvent used, these are: * Petroleum ether •Ether •Ethyl acetate *JV-butanoI v preliminary phytochemical investigation was made to eacn separately. In this respect, ether and ethyl acetate fractions were rf &r<*ra yottJrs rx ^rae fungal content of both the rhizosphere and thizoplane of the infected plants was compared with those of the healthy ones. In this concern there was no significant difference between the rhizosphere of the both types of plants. The causal fungal pathogen of tomato wilt was isolated and i identified to be Fusarium oxysporum. Altemaria alternata and Penicillium digitatum were isolated from rotted tomato and orange fruits respectively. Some Egyptian plants have been studied as controlling agents of some plant fungal diseases.© In our present study, we found that Polygonum salicifolium has good results in this regard. The crude extract of Polygonum was found to have an inhibitory activity against some of pathogenic fungi rnygoHum ***** **** beGtx made of the extract j. „ tn pacri solvent used, these into four parts according to eacn SOL y Some fungi have been isolated from the root of some healthy and wilted tomato plants. The fungal content of both the rhizosphere and rhizoplane of the infected plants was compared with those of the healthy ones. In this concern there was no significant difference between the rhizosphere of the both types of plants. The causal fungal pathogen of tomato wilt was isolated and identified to be Fusarium oxysporum. Ahernaria alternata and Penicillium digitatum were isolated from rotted tomato and orange fruits respectively. Some Egyptian plants have been studied as controlling agents of some plant fungal diseases. In our present study, we found that Polygonum salicifolium has good results in this regard. The crude extract of Polygonum was found to have an inhibitory activity against some of plant pathogenic fungi Fractionations of the crude Polygonum extract have been made using solvents of increasing polarity. The fractionation leads to a partition of the extract into four parts according to each solvent used, these partitions are: * Petroleum ether * Ether * Ethyl acetate * N-butanol A preliminary phytochemical investigation was made to each fraction separately. In this respect, ether and ethyl acetate fractions were observed to have a great similarity in their composition also we Some fungi have been isolated from the root of some healthy and wilted tomato plants. The fungal content of both the rhizosphere and rhizoplane of the infected plants was compared with those of the healthy ones. In this concern there was no significant difference between the rhizosphere of the both types of plants. The causal fungal pathogen of tomato wilt was isolated and identified to be Fusarium oxysporum. Ahernaria alternata and Penicillium digitatum were isolated from rotted tomato and orange fruits respectively. Some Egyptian plants have been studied as controlling agents of some plant fungal diseases. In our present study, we found that Polygonum salicifolium has good results in this regard. The crude extract of Polygonum was found to have an inhibitory activity against some of plant pathogenic fungi Fractionations of the crude Polygonum extract have been made using solvents of increasing polarity. The fractionation leads to a partition of the extract into four parts according to each solvent used, these partitions are: * Petroleum ether * Ether * Ethyl acetate * N-butanol A preliminary phytochemical investigation was made to each fraction separately. In this respect, ether and ethyl acetate fractions were observed to have a great similarity in their composition also we Summary investigated that the two fractions have about the same picture on the TLC in a different solvent systems. Studying the effects of these different fractions at a certain concentration on some plant pathogenic fungi showed that the inhibitory effect of each fraction was differ from the other. Petroleum ether and butanol fractions have no activity against any of the tested organisms while ether and ethyl acetate showed great notable activities. So that, ether and ethyl acetate fractions were the active parts of Polygonum extract. By studying the linear growth, dry weight, sugar and protein contents and enzyme activities of the three fungi isolated from tomato and orange plants we found that the ether-ethyl acetate fraction of the plant extract has a remarkable activity as it causes a reduction in linear growth, dry weight, sugar and protein contents and enzyme activities in the mat of the examined fungi. By making some application experiments, we found that, the ether-ethyl acetate fraction has an inhibitory effect on F. oxysporum the causal agent of tomato wilt, also it enhanced the growth of the cultivated tomato plants more than in the case of control despite of being inoculated with this pathogenic fungi. It also undergoes the effect of the post-harvest pathogens (A. alternata and P. digitatum) as shown when compared with the infected fruits. These active fractions undergo a separation of their active compounds this resulted in an isolation of three compounds identified using UV, H1 NMR, 13C NMR, H-H COSY, HMQC and HMBC spectra these compounds were: *Quercetin aglycone * Mixture of quercetin 3-0-jff-D-glucoside (isoquercetin) and quercetin 3-0-j8-D-galactoside (hyperoside Querectin is one of the flavonoidal compounds that were recorded to have an inhibitory activity against some pathogenic microorganisms. the mic of these compounds was recorded. |