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Abstract
This work was performed to study the different parts of gastrointestinal tract mucosa in adult albino rats with concentration on the goblet and enteroendocrine cells Material and methods Fifteen adult male albino rats of three to six months old age were used, the animals were anaesthetized with ethyl ether, labarotomy was done and the gastrointestinal tract of the rat was divided into eight portions according to general classification of mammalian GIT. Samples from fundus, pylorus, duodenum, jejunum, ileum and colon were taken, each of which was 1 cm long and were fixed in 10 % buffered formalin and processed for paraffin sections which were stained with H&E, Alcian blue and immunohistochemical stain for chromogranin A; a marker of enteroendocrine cells and examined with light microscope. The mean value of area% of CgA immunoreactive cells were calculated by the help of image analysis and compared with each others for the sex segments to be studied.
Samples from pylorus, duodenum and colon removed from animal and were placed on a dental wax in a DROP of the primary fixative and cut them with sharp razor into minute small pieces (1 mm) and fixed immediately in a buffered gluteraldehyde solution at PH (7.4) from 2 to 24 hours in a refrigerator at 4°C then processed for transmission electron microscope.