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Abstract The presem work was sub)ecte to study pastur1(osis as a cause o pneumonia in sheap regarding to isolation of the causative agent, the most suitable antibiotic to treat infected animals as well as preparotion and evaluation of a specific combined vaccine among the control of such disease . The experimental results showed that 1) Symptoms of pasteureffosis were detected in 20 % of a sheep flock with recorded deaths on the 1st and 2 nd days in the early stages and on one week or move in the late stages of the disease . The post mortom findings were characteristic for pastearella multocida 2) Pa.steureffa multocida was isolated from nasal swabs in the ratio of 6 % in case of healthy animals and 10% in case of diseased ones . It was also isolated from healthy lungs (3.3%) and diseased lungs (8%). 3) Biotyping of the obtained isolates using the different biochemical tests revealed that out of 56 paste urelia isolates, 7 isolates were belonged to the capsular group A five of them were somatic biotype 1 and two of the somatic type 3. other 34 isolates were belonged to the capsular group B and somatic biotype 6 while 9 isolates were foun belonged to the capsulafr group D, five of them were somatic biotype 1, one From the somotic biotype 2 and three of somatic biotype 4. six isolates were untypable. 4) Antibiotic sensetivity test revealed that all isolates of pasteuretia multocid ii were highly sensetive to Ampicillin, Choloramphenicol and Gentamycin of obtained Pa$teurelua muftocida isolates 12.1 % were found sensetive to penicillin 0 and Oxytetracycline, 85,7 % to Nalidixic acid 53.5% , to tetracyclince, while only 16.7% were found sensetive to Trimethoxazol plus sulphamethxol |